Science Focus Area: Lawrence Livermore National Laboratory
- Principal Investigator: Jennifer Pett-Ridge1
- Co-Investigators: Steve Blazewicz1, Yongqin Jiao1, Jeff Kimbrel1, Stephanie Malfatti1, Karis McFarlane1, Erin Nuccio1, Brian Souza1, Peter Weber1, Mavrik Zavarin1, Mary Firestone2, Jill Banfield2, Bruce Hungate3, Paul Dijkstra3, Christina Schaedel3, Ben Koch3, Egbert Schwartz3, Eoin Brodie4, Peter Nico4, Jinyun Tang4, Matt Sullivan5, Ljiljana Paša-Tolic6
- Participating Institutions: 1Lawrence Livermore National Laboratory, 2University of California–Berkeley, 3Northern Arizona University, 4Lawrence Berkeley National Laboratory, 5Ohio State University, 6Pacific Northwest National Laboratory
- Collaborations: Peter Kennedy, University of Minnesota; Asmeret Asefaw Berhe, University of California–Merced; Lawrence Berkeley National Laboratory and the DOE Systems Biology Knowledgebase (KBase)
- Project Website: https://sc-programs.llnl.gov/biological-and-environmental-research-at-llnl/soil-microbiome
- KBase Collaboration: Foundation for Understanding and Prediction of Microbial and Viral Ecogenomics in Soil
Summary

Water Dynamics Across Scales. The Microbes Persist SFA at Lawrence Livermore National Laboratory (LLNL) seeks to gain a foundational and predictive understanding of the interacting biological, biogeochemical, and physical factors that control soil carbon turnover and persistence. Because of the vast complexity of soils, the multi-institutional team includes experts in a number of disciplines such as soil microbiology, ecophysiology and biogeochemistry, metagenomics and viral ecology, organic matter–mineral chemistry, isotope and compound-specific mass spectrometry, and multiscale modeling. [Courtesy LLNL]
- Apply stable isotope probing (SIP) metagenomics to delineate how changing water regimes shape the activity of individual microbial populations and expression of ecophysiological traits that affect the fate of microbial and plant carbon.
- Identify and quantify mechanisms of mortality in the soil microbiome (focusing on phage lysis, bacterial predators, and water stress) and their contributions to carbon turnover and the biochemistry of microbial residues.
- Measure how the soil microbiome and its products (cell envelope, extracellular polymeric substances, exo-enzymes) interact with contrasting mineral assemblages to control both short- and long-term soil carbon persistence.
- Synthesize genome-scale ecophysiological trait data, population-specific growth and mortality information, and SOM chemistry to build models of microbial functional guilds and SOM turnover, and to predict the long-aspired connection between soil microbiomes and the fate of soil carbon.