The Joint BioEnergy Institute ( JBEI) is working to convert nonfood bioenergy crops into economically viable, carbon-neutral biofuels and bioproducts currently derived from petroleum, as well as many other bioproducts that are not efficiently produced from petroleum.
Ultimately, JBEI aims to:
- Develop robust and sustainable bioenergy crops for different regions.
- Advance basic understanding of plant cell walls, environmental interactions, biomass recalcitrance, and microbial physiology and metabolism.
- Establish predictive biosystems design tools for plants, microbes, and enzymes.
- Develop technologies for feedstock-agnostic biomass deconstruction that liberate high yields of sugars and lignin-derived intermediates suitable for bioconversion to biofuels and bioproducts.
- Enable production of drop-in biofuels and novel bioproducts.
- Promote an inclusive bioenergy enterprise based on diversity and equity.
Research Focus AreasJBEI’s research is establishing the scientific knowledge and new technologies in sustainability, feedstock development, deconstruction and separation, and conversion that are needed to transform the maximum amount of carbon available in bioenergy crops into biofuels and bioproducts. When fully scaled, these advances will enable the production of affordable and sustainable replacements for petroleum-derived gasoline, diesel, jet fuel, and bioproducts. Additionally, JBEI is developing new approaches, technologies, and software to increase sample throughput, decrease reagent use via microfluidics, increase measurement fidelity, and reduce assay time in biofuels research. These analytical technologies provide the high-quality data needed to feed machine-learning algorithms that can systematically direct metabolic engineering processes.
Sustainability. JBEI seeks to ensure that new bioenergy crops and conversion processes are low-cost, low-input, scalable, and resilient. By combining systems-level economic and environmental models with experimental results, JBEI develops performance goals and prioritizations for biofuels and bioproducts produced from sugars, ligninderived intermediates, and in planta intermediates. Techno-economic and life-cycle assessment models integrated with high-throughput experimental pipelines enable rapid iteration and improvement of production processes. Multisite field tests of bioenergy crops, along with agroecosystem models, provide insights into plant-microbe interactions, crop yields, and emissions impacts across a variety of soil and climate conditions.Feedstock Development. JBEI engineers field test sorghum bioenergy crops, with some additional research on poplar, to validate high biomass yield and low susceptibility to disease and drought. To support engineering efforts, JBEI is developing a suite of plant biosystem design tools and improved methods for sorghum transformation. JBEI is also improving the fundamental understanding of plant biology, with a focus on biomass composition, cell wall structure, and plant-microbe interactions. Bioenergy crops developed by JBEI are tailored for facile biomass deconstruction into sugars and lignin-derived intermediates and subsequent microbial conversion into biofuels and bioproducts. Bioenergy crops are also engineered to produce bioproducts, such as precursors of plastic polymers, directly in plant biomass. Deconstruction and Separation. JBEI is developing an integrated, feedstock-agnostic biomass deconstruction process that pretreats biomass with renewable and biocompatible ionic liquids and deep eutectic solvents, as well as optimized enzyme mixtures, which then depolymerize plant polysaccharides and lignins into bioavailable intermediates, such as glucose, xylose, and p-coumarate. This integrated “one-pot” deconstruction process liberates high yields (as much as 90% or more) of the sugars and lignin-derived intermediates contained in bioenergy crops. JBEI’s sorghum and poplar lines are suitable for conversion into biofuels and bioproducts using these technologies and feeding the intermediates to JBEI-engineered microbes. The work includes exploration of ecosystems and microbial communities that naturally produce deconstruction enzymes targeted for optimization. In addition, JBEI researchers are developing predictive biomass deconstruction tools that will enable efficient design of affordable and scalable deconstruction processes.
Conversion. JBEI is engineering microbes, such as Pseudomonas putida and Rhodosporidium toruloides, that fully metabolize the sugars and aromatics generated during the deconstruction process to produce a variety of targeted biofuels and bioproducts at industrially relevant titers, rates, and yields. These are products that otherwise would be made from petroleum using traditional chemistry. JBEI has developed advanced tools to design new biological routes and engineer microbial metabolic pathways and genomes guided by genome-scale models, functional genomics, and data-driven approaches.
To produce drop-in biofuels and bioproducts that directly replace existing products, as well as novel bioproducts with differentiated properties, JBEI has developed a versatile repertoire of biological conversion routes to major biosynthesis products such as terpenes, polyketides, and fatty acids. A prioritized JBEI biofuel target is isoprenol (3-methyl-3-butene1-ol). This valuable gasoline blendstock is convertible to the sustainable aviation fuel dimethylcyclooctane (DMCO) and is an important precursor of several commodity chemicals such as isoprene. JBEI has also pioneered the development of novel advanced biofuels such as fuelimycin A, a high-energy-density compound and potential rocket fuel derived from polyketide synthase.
JBEI is committed to transformative research and innovation that results in economic and performance step changes for biomanufacturing. Through its advisory committee, biobased targets council, and continual outreach efforts, JBEI cultivates relationships with industry thought leaders. Robust communication with the private sector ensures that JBEI’s intellectual property (IP) is both cutting edge and industrially compelling, resulting in a rigorous commercialization pipeline consisting of multiple start-up companies, licensing agreements, and strategic partnerships.Lawrence Berkeley National Laboratory manages all JBEI-related partnership agreements and IP, regardless of which partner institution owns the IP. In addition, JBEI employs a director of commercialization who coordinates all JBEI industry interactions. These structures enable industry to work with a single institution and point of contact to access all that JBEI has to offer.
Education and Outreach
The goal of JBEI’s education and outreach program is to share JBEI’s research efforts and successes with the public and the bioenergy research sector, advocate for biofuels and bioproducts research, and highlight the benefits of publicly funded basic science research. The program also serves as an educational resource for next-generation work forces in science, technology, engineering, mathematics (STEM); biomanufacturing; and clean energy. JBEI is committed to creating a culture of inclusion that focuses on equity and valuing diversity in the workplace and surrounding community. This approach is key to attracting and engaging the brightest minds and advancing scientific excellence and groundbreaking innovations.
JBEI’s current efforts include outreach and training opportunities for the future bioeconomy workforce, including educational programs and internships for K-12, undergraduate, and graduate students. JBEI’s key initiative, the Introductory College Level Experience in Microbiology (iCLEM) program, provides under-resourced San Francisco Bay Area high school students with paid summer internships conducting scientific research. The iCLEM program is nationally recognized, with nearly all alumni attending college and more than 80% majoring in STEM. Each year, iCLEM also extends its reach by hosting teachers and teachers-in-training who incorporate iCLEM lessons into their curricula and develop new curriculum modules.
- Lawrence Berkeley National Laboratory (Berkeley, California; lead institution)
- Brookhaven National Laboratory (Upton, New York)
- Georgia Institute of Technology (Atlanta)
- Iowa State University (Ames)
- Lawrence Livermore National Laboratory (Livermore, California)
- Northwestern University (Evanston, Illinois)
- Pacific Northwest National Laboratory (Richland, Washington)
- Sandia National Laboratories (Livermore, California; Albuquerque, New Mexico)
- The University of Adelaide (Australia)
- University of California—Berkeley
- University of California —Davis
- University of California—San Diego
- University of California—Santa Barbara
- 5885 Hollis St.
Emeryville, CA 94608
- (510) 486-7315
- Jay Keasling: JDKeasling@lbl.gov