Microorganisms play key roles in soil carbon turnover and stabilization of persistent organic matter via their metabolic activities, cellular biochemistry, and extracellular products. Microbial residues are also thought to be primary ingredients of soil organic matter (SOM), a pool critical to Earth’s soil health and climate. The central hypothesis of this SFA is that microbial cellular-chemistry, functional potential, and ecophysiology fundamentally shape soil carbon persistence—this will be characterized via stable isotope probing (SIP) of genome resolved metagenomes. Studies will focus on soil moisture as a 'master controller' of microbial activity and mortality, since altered precipitation regimes are predicted across the temperate U.S.
The LLNL Soil Microbiome SFA’s ultimate goal is to determine how microbial soil ecophysiology, population dynamics, and microbe-mineral-organic matter interactions regulate the persistence of microbial residues under changing moisture regimes.
Laboratory Research Manager: Henry Shaw1
Lead Investigator: Jennifer Pett-Ridge1
LLNL SFA Team: Steve Blazewicz1, Yongqin Jiao1, Stephanie Malfatti1, Karis McFarlane1, Erin Nuccio1, Brian Souza1, Ben Stewart1, Rhona Stuart1, Peter Weber1, Mavrik Zavarin1
Collaborators: Eoin Brodie2, Peter Nico2, Jinyun Tang2, Mary Firestone3, Jill Banfield3, Bruce Hungate4, Paul Dijkstra4, Christina Schaedel4, Ben Koch4, Matt Sullivan3, Ljiljana Paša-Tolic6
Postdocs/Students: Rachel Neurath1,3, Keith Morrison1, Evan Starr3, Alexa Nicholas3
Participating Institutions: 1Lawrence Livermore National Lab (LLNL), 2Lawrence Berkeley National Lab (LBNL), 3University of California Berkeley (UCB), 4Northern Arizona University (NAU), 5Ohio State University (OSU), 6Environmental and Molecular Sciences Laboratory/Pacific Northwest National Lab (EMSL/PNNL)