Genomic Science Program

Genomic Science Program Research Awards

---

Molecular and Cellular Systems: A Research Program for Identification and Characterization of Protein Complexes

Oak Ridge National Laboratory
Michelle Buchanan, Principal Investigator
Pacific Northwest National Laboratory
Steve Wiley, Principal Investigator
$23.4 million over 5 years
Funded: FY2002

Research Partners:

• Argonne National Laboratory
  Carol Giometti, Co-Principal Investigator
• Sandia National Laboratories
  Malin Young, Co-Principal Investigator
• University of North Carolina at Chapel Hill
  Morgan Giddings, Co-Principal Investigator

This team, led by Oak Ridge and Pacific Northwest National Laboratories, will develop and use the technologies needed to identify and characterize the complete set of multiprotein complexes, the molecular machines of life, within a microbial cell. The research will focus on two microbes—one that plays a significant role in earth's carbon cycle and another with an ability to clean up metals in contaminated soil.

Website

Abstracts

• 2005 Contractor-Grantee Workshop
  • Oak Ridge National Laboratory and Pacific Northwest National Laboratory (PDF, 157 kb)
  • Center for Molecular and Cellular Systems: High-Throughput Identification and Characterization of Protein Complexes
  • High-Throughput Analysis of Protein Complexes in the Center for Molecular and Cellular Systems
  • Investigating Gas Phase Dissociation Pathways of Crosslinked Peptides: Application to Protein Complex Determination
  • Center for Molecular and Cellular Systems: Statistical Screens for Datasets from High- Throughput Protein Pull-Down Assays
  • Center for Molecular and Cellular Systems: Analysis and Visualization of Data from a High-Throughput Protein Complex Identification Pipeline Using Modular and Automated Tools
• 2004 Contractor-Grantee Workshop
  • Establishment of Protocols for the High Throughput Analysis of Protein Complexes at the Center for Molecular and Cellular Systems
  • Isolation and Characterization of Protein Complexes from Shewanella oneidensis and Rhodopseudomonas palustris
  • Bioinformatics and Computing in the Genomics:GTL Center for Molecular and Cellular Systems - LIMS and Mass Spectrometric Analysis of Proteome Data
  • Advanced Computational Methodologies for Protein Mass Spectral Data Analysis
  • High-Throughput Cloning, Expression and Purification of Rhodopseudomonas palustris and Shewanella oneidensis Affinity Tagged Fusion Proteins for Protein Complex Isolation
• 2003 Contractor-Grantee Workshop
  • Bioinformatics and Computing in the Genomes to Life Center for Molecular and Cellular Systems
  • Mass Spectrometry in the Genomes to Life Center for Molecular and Cellular Systems
  • Genomes to Life Center for Molecular and Cellular Systems: A Research Program for Identification and Characterization of Protein Complexes
  • New Approaches for High-Throughput Identification and Characterization of Protein Complexes
  • Automation of Protein Complex Analyses in Rhodopseudomonas palustris and Shewanella oneidensis