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Plant Feedstock Genomics for Bioenergy

DOE

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USDA and DOE Fund Ten New Projects in 2014 for Biomass Genomics Research

The U.S. Department of Energy's Office of Science, Office of Biological and Environmental Research, and the U.S. Department of Agriculture (USDA) National Institute of Food and Agriculture’s Agriculture and Food Research Initiative* have jointly selected ten projects for awards totaling $12.6 million for biobased-fuel research. These awards continue a commitment begun in 2006 to conduct fundamental research in biomass genomics that will establish a scientific foundation to facilitate and accelerate the use of woody plant tissue for bioenergy and biofuel.

2014 Awards

tree and grass logoIn 2014, DOE will provide $10.6 million in funding over 3 years, while USDA will award $2 million over 3 years.

Coordinated Genetic Improvement of Bioenergy Sorghum for Compositional and Agronomic Traits

  • Patrick Brown, University of Illinois, Urbana-Champaign, $1,339,800
  • DOE-BER funded project

Goal: Discover and characterize novel genetic variants that affect lignocellulosic composition and saccharification yield in bioenergy feedstock grasses without compromising agronomic performance. This project will characterize genetic variation in compositional and agronomic traits in a panel of 600 diverse sorghum inbreds and identify useful traits and variants that will guide and accelerate the genetic improvement of both bioenergy sorghum and closely related perennial grasses.

Abiotic Stress Networks Converging on FT2 to Control Growth in Populus

  • Amy Brunner, Virginia Polytechnic Institute and State University, Blacksburg $1,430,400
  • DOE-BER funded project

Goal: Uncover divergent and convergent regulatory networks that control growth responses to daylength and nutrient stress in poplar. Regulation of growth and dormancy by such seasonal and episodic environmental factors is of central importance to productivity in temperate tree species. This project will characterize genome-wide gene expression changes in response to daylength and nutrient stress and identify protein-protein and protein-DNA networks that are centered on FT2, a key integrator of multiple abiotic signaling pathways in Populus.

Exploiting Natural Diversity to Identify Alleles and Mechanisms of Cold Adaptation in Switchgrass

  • Robin Buell, Michigan State University, East Lansing $1,000,000
  • USDA-NIFA funded project

Goal: Identify metabolites, alleles, transcripts, and regulatory RNAs associated with cold hardiness in switchgrass that will advance understanding of the biochemical, physiological, and molecular mechanisms for cold adaptation and provide molecular tools to improve breeding efficiency.

A Novel Poplar Biomass Germplasm Resource for Functional Genomics and Breeding

  • Luca Comai, University of California, Davis $1,274,800
  • DOE-BER funded project

Goal: Further develop the poplar indel germplasm collection and use it to investigate the role of gene dosage in poplar hybrid performance and contribution to bioenergy traits. This project will catalog dosage variation in ~500 Populus deltoides × P. nigra F1 individuals, use field trials to characterize variation for traits central to sustainable production of biomass with optimal feedstock properties, and identify specific regulatory or functional gene modules underlying phenotypes of interest, ultimately to produce new cultivars directly usable for bioenergy applications.

Genetic Dissection of AM Symbiosis to Improve the Sustainability of Feedstock Production

  • Maria Harrison, Boyce Thompson Institute for Plant Research, Ithaca, NY $864,400
  • DOE-BER funded project

Goal: Understand the genetic bases of arbuscular mycorrhizal (AM) symbiosis in feedstocks through studies of a model feedstock species, Brachypodium distachyon, and sorghum, a feedstock species. This project will utilize Brachypodium to evaluate the function of candidate proteins that potentially control development of the symbiosis and symbiotic P and N transport, and then evaluate AM symbiosis in diverse sweet and cellulosic (bioenergy) sorghum lines. This research will inform feedstock breeding programs and enhance the sustainability of feedstock production.

Advancing Field Pennycress as a New Oilseed Biodiesel

  • Michael Marks, University of Minnesota, Minneapolis $1,000,000
  • USDA-NIFA funded project

Goal: Genetically improve the agronomic traits of field pennycress (Thlaspi arvense L) for its use as a new winter annual oilseed/meal/cover crop in the Upper Midwest.

Biofuels in the Arid West: Germplasm Development for Sustainable Production of Camelina Oilseed

  • John McKay, Colorado State University, Fort Collins $1,487,200
  • DOE-BER funded project

Goal: Facilitate the development of Camelina as an oilseed feedstock crop that can be grown on marginal farmland with relatively low fertilizer inputs and no irrigation. Camelina has many optimal qualities as an oilseed feedstock, but its performance as a fuel with minimal processing can be improved. Leveraging the newly available genome sequence of Camelina sativa, this project will use forward and reverse genetics and natural variation to combine optimal qualities in Camelina as an oilseed feedstock for the Great Plains and Western United States.

The Brachypodium ENCODE Project—From Sequence to Function: Predicting Physiological Responses in Grasses to Facilitate Engineering of Biofuel Crops

  • Todd Mockler, Donald Danforth Plant Science Center, St. Louis, MO $1,498,600
  • DOE-BER funded project

Goal: Identify and characterize the functional elements associated with progressive drought response in the Brachypodium distachyon genome sequence and develop integrated genome feature maps that will enable advanced modeling of complex pathways in plants. Using a model grass, the Brachypodium ENCODE (for Encyclopedia of DNA Elements) project will elucidate the molecular mechanisms and gene regulatory networks underlying drought stress, information which will aid basic and applied research on a wide range of bioenergy grasses and accelerate deployment of improved bioenergy grass feedstocks.

Genomics of Energy Sorghum's Water Use Efficiency / Drought Resilience

  • John Mullet, Texas A&M University, College Station $1,230,600
  • DOE-BER funded project

Goal: Increase the water use efficiency, drought resilience, and yield of high biomass energy Sorghum and other C4 bioenergy grasses. This project will use field analysis to identify traits and molecular responses that improve water use efficiency and drought resilience of energy Sorghum and characterize genetic variation, and then test the utility of modulating these traits in energy Sorghum hybrids through marker-assisted breeding.

Quantifying Phenotypic and Genetic Diversity of Miscanthus sacchariflorus to Facilitate Knowledge of Directed Improvement of M.×giganteus (M. sinensis × M. sacchariflorus) and Sugarcane

  • Erik Sacks, University of Illinois, Urbana-Champaign $1,496,300
  • DOE-BER funded project

Goal: Facilitate the rapid development of Miscanthus as a bioenergy crop by obtaining fundamental knowledge about M. sacchariflorus (Msa) genetic diversity, population structure, and environmental adaptation. This project will conduct field trials with ~600 individuals of Msa from throughout its natural range to evaluate yield potential and adaptation. It will develop molecular markers associated with traits of interest that will enable plant breeders to quickly develop improved biomass cultivars of M×g as well as the closely related sugarcanes and energycanes.

 

Current and Previous USDA DOE Plant Feedstock Awards

*formerly the Cooperative State Research, Education, and Extension Service (CSREES), National Research Initiative

Featuring

Plant Feedstock Genomics for Bioenergy Abstracts [07/14]


Sustainable Bioenergy [05/14]


USDA/DOE Plant Feedstocks Genomics for Bioenergy Program: Project Director / Principal Investigator Meeting [01/14]


Switchgrass Research Group: Progress Report [1/12]


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